Journal: Environmental Health and Toxicology
Article Title: Comparative study of oxidative stress caused by anthracene and alkyl-anthracenes in Caenorhabditis elegans
doi: 10.5620/eht.e2018006
Figure Lengend Snippet: Oxidative stress markers investigated in C. elegans exposed to the concentration close to the water solubility of Ant, 9-MA, and 9,10-DMA for 3 hours. (A) The formation of reactive oxygen species (ROS) detected using the fluorescence intensity by 2,7-dichlorofluoroscein diacetate staining, (B) the percentage of superoxide dismutase (SOD) activity, (C) total glutathione, and (D) lipid peroxidation measured as malondialdehyde (MDA) concentration. Error bars denote standard deviations. The letters represent statistical significance ( p <0.05) determined by Duncan's multiple range test. Ant, anthracene; 9-MA, 9-methylanthracene; 9,10-DMA, 9,10-dimethylanthracene.
Article Snippet: The activity of SOD was measured using an Oxiselect TM Superoxide Dismutase Activity Assay kit (cat. STA-340; Cell Biolabs, Inc., San Diego, CA, USA).
Techniques: Concentration Assay, Solubility, Fluorescence, Staining, Activity Assay